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KMID : 1134820190480020189
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Journal of the Korean Society of Food Science and Nutrition
2019 Volume.48 No. 2 p.189 ~ p.197
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Hepatoprotective Effect of Chrysanthemum zawadskii Extract (CZE) in Experimentally Induced Liver Damage Model In Vitro and In Vivo
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Kim Yong-Sang
Kim Seul-Ki
Kwon Da-Ae
Kim Hyun-Kyu
Lee Hak-Sung
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Abstract
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In vitro and in vivo experiments were conducted to examine the effects of an improvement of Chrysanthemum zawadskii extract (CZE) on the function of the liver. The lipid-related biomarkers in the liver were analyzed by RT-PCR using oleic acid (OA)-induced steatotic HepG2 cells. Treatment with 125, 250, and 500 μg/mL CZE decreased the intracellular lipid accumulation compared to the OA alone treatment group. CZE reduced the fatty acid influx to hepatocytes and synthesis through the down-regulation of FAT/CD36, SREBP-1C, and FAS mRNA expression in the steatotic HepG2 cells. In addition, PPARα and CPT-1 mRNA expression in HepG2 cells were increased significantly by a CZE treatment, by up to 46.4% and 28.6%, respectively, compared to the OA alone treatment group due to the activation of beta oxidation. In addition, the hepatoprotective effect of CZE was investigated by conducting blood biochemical and histopathological analysis in vivo on acute liver toxicity induced by acetaminophen (APAP). CZE was administered orally at concentrations of 125, 250, and 500 μg/mL for 5 days after treatment with APAP (350 mg/kg) to induce severe liver injury in mice. In the APAP treated group, the glutamate oxaloacetate transaminase (GOT/AST) and glutamate pyruvate transaminase (GPT/ALT) levels were rapidly elevated in the liver but decreased significantly in the CZE pretreated group. This protective effect of CZE on APAP induced toxicity was consistent with the histopathological examination. The triglyceride (TG) and total cholesterol (TC) levels were significantly lower in the liver tissues of the CZE-pretreated group than in the APAP alone treated group. In addition, hepatic glutathione (GSH) level was significantly increased by the CZE treatment in a dose-dependent manner. These results suggest that a treatment with CZE alleviates the imbalance of the liver lipid metabolism caused by a nonalcoholic fatty liver and suggests that it can help relieve the acute hepatotoxic effect induced by APAP.
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KEYWORD
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Chrysanthemum zawadskii, RT-PCR, nonalcoholic fatty liver, HepG2, acetaminophen (APAP)
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